内容

目次

Part 1 A user's guide: getting started. Part 2 General principles:types of nerve cell cultures, their advantages and limitations; primarydissociated cell cultures of neuronal tissue; characterizing andstudying neuronal cultures. Part 2 Culture of specific cell-types:isolated chick neurons for the study of axonal growth; culturingspinalneurons and muscle cells from xenopus embryos; culturing thelarge neurons of aplysia; tissue culture of mammalian autonomicneurons; methodologies for the culture and experimental use of the PC12rat pheochromocytoma cell line; cell culture of neocortex and basalforebrain from postnatal rats; rat hippocampal neurons in low-densityculture; cerebellar cells in culture; astroglia in culture; tissueculture methods for the study of myelination; organotypic slicecultures of neural tissue.