内容

目次

Polymerase Chain Reaction. Selection of Primers for Polymerase ChainReaction. Direct Radioactive Labeling of Polymerase Chain Reaction Products.Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-StrandedProbes for S1 Nuclease Assays. Nonradioactive Labeling of Polymerase ChainReaction Products. Quantitation and Purification of Polymerase Chain ReactionProducts by High-Performance Liquid Chromatography. Use of Polymerase ChainReaction for Screening Transgenic Mice. Polymerase Chain Reaction Analysis ofDNA from Paraffin-Embedded Tissue. The Use of Polymerase Chain Reaction forChromosome Assignment. Mapping MHC Class II Genes and Disease-Susceptibility.The Use of the Polymerase Chain Reaction and the Detection of AmplifiedProducts. Determination of Loss of Heterozygosity Using Polymerase ChainReaction. Direct Sequencing of Polymerase Chain Reaction Products. Manual andAutomated Direct Sequencing of Product Generated by the Polymerase ChainReaction. Genomic Footprinting by Ligation Mediated Polymerase ChainReaction. RNA Template-Specific Polymerase Chain Reaction (RS-PCR).Quantitative Measurement of Relative Gene Expression in Human Tumors.Identification of Alternatively Spliced mRNAs and Localization of 5' Ends byPolymerase Chain Reaction Amplification. Utilization of Polymerase ChainReaction for Clonal Analysis of Gene Expression. Sequencing DNA AmplifiedDirectly from a Bacterial Colony. Use of Polymerase Chain Reaction to ScreenPhage Libraries. Molecular Cloning of Polymerase Chain Reaction Fragmentswith Cohesive Ends. Rapid (Ligase-Free) Subcloning of Polymerase ChainReaction Products. Use of Polymerase Chain Reaction for Making RecombinantConstructs. In Vitro Recombination and Mutagenesis of DNA. Use of PolymeraseChain Reaction for the Rapid Construction of Synthetic Genes. RecombinantCircle Polymerase Chain Reaction for Site-Directed Mutagenesis. Site-DirectedMutagenesis by Double Polymerase Chain Reaction. Generation of a PolymeraseChain Reaction Renewable Source of Subtractive cDNA. PCR-Based Full-LengthcDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-PairStrategy. Use of Degenerate Oligonucleotide Primers and the Polymerase ChainReaction to Clone Gene Family Members. Single Specific Primer-PolymeraseChain Reaction (SSP-PCR) and Genome Walking. cDNA Cloning by InversePolymerase Chain Reaction. Amplification of Gene Ends from Gene Libraries byPolymerase Chain Reaction with Single-Sided Specificity. Anchoring a DefinedSequence to the 5' Ends of mRNAs. Index.