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Contents: Section I: Detection and Approaches to Live Cell Imaging 1. Fluorescent Protein Tracking and Detection 2. Constructing and Expressing Fluorescent Protein Fusions 3. Micropatterning Cell–Substrate Adhesions Using Linear Polyacrylamide as the Blocking Agent 4. CCD Cameras for Fluorescence Imaging of Living Cells 5. Fluorescence-Perturbation Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, Photoactivation, Photoconversion, and FLIP 6. Imaging Protein State in Cells 7. A Versatile, Multicolor Total Internal Reflection Fluorescence and Spinning-Disk Confocal Microscope System for High-Resolution Live Cell Imaging 8. Confocal Microscopy, Deconvolution, and Structured Illumination Methods 9. Atomic-Force Microscopy for Biological Imaging and Mechanical Testing across Length Scales 10. OMX 11. SPIM 12. First Step for Fluorescence Correlation Spectroscopy for the Live Cell 13. Tracking and Quantitative Analysis of Dynamic Movements of Cells and Particles 14. Imaging Techniques for Measuring Cell Materials Properties 15. Computational Image Analysis of Cellular Dynamics: A Case Study Based on Particle Tracking 16. Survey of Software Packages Used for Image Analysis 17. High-Throughput Microscopy Using Live Mammalian Cells Section II: Imaging of Live Cells and Organisms 18. In Vivo Imaging of Mammalian Cells 19. Live Cell Imaging of Yeast 20. Live Imaging of Caenorhabditis elegans 21. Live Cell Imaging of Plants 22. Time-Lapse Cinematography in Living Drosophila Tissues 23. 3D Imaging: Spinning Disk Confocal Microscopy for Imaging Tumor Models 24. Single-Cell Imaging in Animals 25. Live Cell and Tissue-Based Correlative Fluorescence and Election Microscopy 26. Intravital Microscopy of Normal and Diseased Tissues in the Mouse 27. PI Signaling 28. Development of Mammalian Cell Lines with Lac Operon–Tagged Chromosomes 29. Imaging Gene Expression in Living Cells 30. Studying Mitosis in Living Cells 31. Imaging Intermediate Filament Proteins in Living Cells 32. Monitoring the Motile Properties of Microtubules and Their Associated Proteins 33. Imaging of Membrane Systems and Membrane Traffic in Living Cells 34. Imaging Live Cells Under Mechanical Stress 35. Imaging Single Molecules Using Total Internal Reflection Microscopy 36. Cellular Imaging Using Total Internal Reflection Fluorescent Microscopy (TIRFM) 37. Visualization and Quantification of Single RNA Molecules in Living Cells