Difference Gel Electrophoresis:Methods and Protocols (Methods in Molecular Biology, Vol. 1664) '17
目次
Part I: Introduction 1. Two Dimensional Gel Electrophoresis and 2D-DIGE Paula Meleady Part II: Difference Gel Electrophoresis 2. Comparative DIGE Proteomics Kay Ohlendieck 3. 2D-DIGE and Fluorescence Image Analysis Elisa Robotti and Emilio Marengo 4. DIGE Analysis Software and Protein Identification Approaches Abduladim Hmmier and Paul Dowling Part III: DIGE Applications 5. Native DIGE: Efficient Tool to Elucidate Protein Interactomes Diksha Dani and Norbert Dencher 6. Comparative Two-dimensional Fluorescence Gel Electrophoresis Doreen Ackermann and Simone König 7. DIGE-based Phosphoproteomic Analysis Taras Stasyk and Lukas Huber 8. DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue Paul Dowling 9. Comparative 3-sample DIGE Analysis of Skeletal Muscles Kay Ohlendieck 10. DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples Sandra Murphy and Paul Dowling Part IV: Comparative DIGE Tissue Proteomics 11. DIGE Analysis of Human Tissues Cecilia Gelfi and Daniele Capitanio 12. DIGE Analysis of Animal Tissues Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, and Giuliano Elia 13. Rapid 2D DIGE Proteomic Analysis of Mouse Liver Shotaro Kamata and Isao Ishii 14. Proteomic Analysis of Lung Tissue by DIGE Jarlath Nally and Simone Schuller 15. Comparative Testis Tissue Proteomics using 2-dye versus 3-dye DIGE Analysis Ashling Holland 16. DIGE Analysis of Fish Tissues Joanna Nynca, Mariola Dietrich and Andrzej Ciereszko Part V: Auxillary DIGE Methods 17. Protein Digestion for DIGE Analysis Sandra Murphy and Kay Ohlendieck 18. Subcellular Fractionation for DIGE-based Proteomics Sandra Murphy 19. DIGE Analysis of Immunodepleted Plasma Paul Dowling and Kay Ohlendieck Part VI: DIGE Verification Analysis 20. Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays Andrew Dowd 21. Enzyme Assay Methods to Validate DIGE Proteomics Data Andrew Dowd 22. Immunoblot Analysis of DIGE-based Proteomics Martin Landsberger and Heinrich Brinkmeier 23. Immunofluorescence Microscopy for DIGE-based Proteomics Rustam Mundegar, Margit Zweyer, and Dieter Swandulla
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