目次

1. Protein Engineering - Past, Present, and Future Stefan Lutz and Samantha M. Iamurri   Part I 2. (Semi)rational Protein Design Ivan V. Korendovych   3. Computational Analysis of Protein Tunnels and Channels Jan Brezovsky, Barbora Kozlikova, and Jiri Damborsky   4. YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations Henrik Land and Maria Svedendahl Humble   5. A Computational Library Design Protocol for Rapid Improvement of Protein Stability - FRESCO Hein J. Wijma, Maximilian J.L.J. Fürst, and Dick B. Janssen   6. Directed Evolution of Proteins Based on Mutational Scanning Carlos G. Acevedo-Rocha, Matteo Ferla, and Manfred T. Reetz   Part II 7. A Brief Guide to the High-throughput Expression of Directed Evolution Libraries Ana Luísa Ribeiro, Mario Mencía, and Aurelio Hidalgo   8. Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers Sandy Schmidt, Mark Dörr, and Uwe T. Bornscheuer   9. Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification Javier Santos-Aberturas, Mark Dörr, and Uwe T. Bornscheuer   10. Functional Analysis of Membrane Proteins Produced by Cell-Free Translation Srujan Kumar Dondapati, Doreen A Wüstenhagen, and Stefan Kubick     PART III 11. Practical Considerations Regarding the Choice of the Best High-throughput Assay Carolin Mügge and Robert Kouristb   12. High-throughput Screening Assays for Lipolytic Enzymes Alexander Fulton, Marc R. Hayes, Ulrich Schwaneberg, Jörg Pietruszka, and Karl-Erich Jaeger   13. Continuous High-throughput Colorimetric Assays for α-transaminases Egon Heuson, Jean-Louis Petit, Franck Charmantray, Véronique de Bérardinis, and Thierry Gefflaut   14. Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases Isabel Pardo and Susana Camarero   15. Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions Min Liu, Lidan Ye, and Hongwei Yu   16. Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation Mark Dörr and Uwe T. Bornscheuer   17. Solid Phase Agar Plate Assay for Screening Amine Transaminases Martin S. Weiß, Uwe T. Bornscheuer, and Matthias Höhne   18. Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics Fabrice Gielen, Pierre-Yves Colin, Philip Mair, and Florian Hollfelder   19. Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display Christian Schröter, Simon Krah, Jan Beck, Doreen Könning, Julius Grzeschik, Bernhard Valldorf, Stefan Zielonka, and Harald Kolmar   20. Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus Jörg Claren, Thomas Schwab, and Reinhard Sterner