Cancer Stem Cells:Methods and Protocols (Methods in Molecular Biology, Vol. 1692) '17
目次
1. Introduction to Cancer Stem Cells: Past, Present, and Future David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi, Chitra Venugopal, and Shiela K. Singh 2. Surface Markers for the Identification of Cancer Stem Cells Vinod Gopalan, Farhadul Islam, and Alfred King-yin Lam 3. The Role of CD44 and Cancer Stem Cells Liang Wang, Xiangsheng Zuo, Keping Xie, and Daoyan Wei 4. Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay Luigi Mele, Davide Liccardo, and Virginia Tirino 5. Isolation of Cancer Stem Cells by Side Population Method Masayuki Shimoda, Masahide Ota, and Yasunori Okada 6. Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, and Geeta Mehta 7. In Vitro Tumorigenic Assay: The Tumor Spheres Assay Hui Wang, Anna Paczulla, Martina Konantz, and Claudia Lengerke 8. In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells Vijayalakshmi Rajendran and Mayur Vilas Jain 9. Xenograft as In Vivo Experimental Model Manuela Porru, Luca Pompili, Carla Caruso, and Carlo Leonetti 10. How to Assess Drug Resistance in Cancer Stem Cells Maria Laura De Angelis, Ruggero De Maria, and Marta Baiocchi 11. Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells Meenakshi Upreti 12. Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML) Anna Di Biase, Amanda K. Miles, and Tarik Regad 13. Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System Magdalena E. Buczek, Stephen P. Reeder, and Tarik Regad 14. Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform Hiromichi Kawasaki, Angela Lombardi, and Michele Caraglia 15. Assessing DNA Methylation in Cancer Stem Cells Sudipto Das, Bruce Moran, and Antoinette S. Perry 16. Histones Acetylation and Cancer Stem Cells (CSCs) Vivian Petersen Wagner, Manoela Domingues Martins, and Rogerio Moraes Castilho 17. Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, and Renato Franco 18. Circulating Tumor Cells Sebastián A. García, Jürgen Weitz, and Sebastian Schölch
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