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Preface…Table of Contents…Contributing Authors…1. Polyamines in the Context of Metabolic NetworksWegi Wuddineh, Rakesh Minocha, and Subhash C. Minocha2. Molecules for Sensing Polyamines and Transducing their Action in PlantsTomonobu Kusano, GHM Sagor, and Thomas Berberich3. Polyamine Metabolism Responses to Biotic and Abiotic StressFernando M. Romero, Santiago J. Maiale, Franco R. Rossi, Maria Marina, Oscar A. Ruiz, and Andrés Gárriz4. Thermospermine: An Evolutionary Ancient but Functionally New Compound in PlantsTaku Takahashi5. Determination of Polyamines by Capillary Electrophoresis using Salicylaldehyde-5-Sulfonate as a Derivatizing ReagentTakashi Kaneta6. Detection of Thermospermine and Spermine by HPLC in PlantsTaku Takahashi, Ayaka Takano, and Jun-Ichi Kakehi7. Development of Amine-Oxidase Based Biosensors for Spermine and Spermidine AnalysisCristina Tortolini, Gabriele Favero, and Franco Mazzei8. Identification of Branched-Chain Polyamines in HyperthermophilesRyota Hidese, Wakao Fukuda, Masaru Niitsu, and Shinsuke Fujiwara9. Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass SpectrometryLaura Torras-Claveria, Jaume Bastida, Francesc Viladomat, and Antonio F. Tiburcio10. Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-PhthalaldehydeYongqing Hou, Xilong Li, Zhaolai Dai, Zhenlong Wu, Fuller W. Bazer, and Guoyao Wu11. Determination of Arginine and Ornithine Decarboxylase Activities in PlantsRubén Alcázar and Antonio F. Tiburcio12. Determination of S-Adenosylmethionine Decarboxylase Activity in PlantsRubén Alcázar and Antonio F. Tiburcio13. Determination of Copper Amine Oxidase Activity in Plant TissuesRiccardo Angelini, Alessandra Cona, and Paraskevi Tavladoraki14. Determination of di-/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation AssayPanagiotis N. Moschou15. Pentamine as a Substrate for Measuring Spermine Oxidase ActivityKoichi Takao and Yoshiaki Sugita16. Spectrophotometric Quantification of Reactive Oxygen, Nitrogen and Sulfur Species in Plant SamplesChrystalla Antoniou, Andreas Savvides, Egli Georgiadou, and Vasileios Fotopoulos17. Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase AssayTejaswani Saragadam and Narayan S.Punekar18. Determination of Transglutaminase Activity in PlantsS. Del Duca, P.LR. Bonner, I. Aloisi, D. Serafini-Fracassini, and G. Cai19. Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy during Organogenic Nodule Formation in HopAna Margarida Fortes and Jose M. Seguí-Simarro20. Analysis of the Intracellular Localization of Transiently Expressed and Fluorescently Labeled Copper-Containing Amine Oxidases, Diamine Oxidase and N-Methylputrescine Oxidase in Tobacco, using an Agrobacterium infiltration ProtocolTsubasa Shoji21. Techniques used for Functional Characterization of Polyamine TransportersClaudio A. Pereira, Melisa Sayé, Chantal Reigada, and Mariana R. Miranda22. Quantitative Trait Loci for Root Growth Response to Cadaverine in ArabidopsisNicole M. Gibbs, Laura Vaughn Rouhana, and Patrick H. Masson23. Methods Related to Polyamine Control of Cation Transport across Plant MembranesIsaac Zepeda-Jazo and Igor Pottosin24. Analysis of DNA Methylation Content and Patterns in PlantsAndreas Finke, Wilfried Rozhon, and Ales Pecinka25. Investigating Ornithine Decarboxylase Post-Transcriptional Regulation via a Pulldown Assay using Biotinylated TranscriptsAnh Mai and Shannon L. Nowotarski26. Analysis of Cotranslational Polyamine Sensing during Decoding of ODC Antiyme mRNAR. Palanimurugan, Daniela Gödderz, Leo Kurian, and R. Jürgen Dohmen27. Modulation of Protein Synthesis by Polyamines in Mammalian CellsKeiko Kashiwagi, Yusuke Terui, and Kazuei Igarashi28. Determination of Post-Translational Modifications by 2D-PAGE: Applications to PolyaminesMarta Bitrián, Antonio F. Tiburcio, and Rubén Alcázar29. Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine GeneticsKostadin E. Atanasov, Changxin Liu C, Antonio F. Tiburcio, and Rubén Alcázar30. Transcriptome Analysis of PA Gain and Loss of Function MutantsFrancisco Marco and Pedro Carrasco31. High-Throughput Phenotyping in Plant Stress Response: Methods and Potential Applications to Polyamine FieldD. Marko, N. Briglia, S. Summerer, A. Petrozza, F. Cellini, and R. Iannacone32. Abiotic Stress Phenotyping of Polyamine MutantsT. Berberich, GHM Sagor, and T. Kusano33. Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes during Pathogenic InteractionsF.R. Rossi, F.M. Romero, O.A. Ruíz, M. Marina, and A. Gárriz34. Real-Time In Vivo Monitoring of Reactive Oxygen Species in Guard CellsKy Young Park and Kalliopi A. Roubelakis-Angelakis35. Genome-Wide Association Mapping Analyses Applied to Polyamines
Luis Barboza-Barquero, Paul Esker, and Rubén Alcázar36. Polyamine Metabolism in Climacteric and Non-Climacteric Fruit RipeningAna Margarida Fortes and Patricia Agudelo-Romero37. Application of Polyamines to Maintain Functional Properties in Stored FruitsMaría Serrano and Daniel Valero38. Acrolein: An Effective Biomarker for Tissue Damage Produced from PolyaminesKazuei Igarashi, Takeshi Uemura, and Keiko Kashiwagi39. Polyamines and CancerElisabetta Damiani and Heather M. Wallace40. Potential Applications of Polyamines in Agriculture and Plant BiotechnologyAntonio F. Tiburcio and Ruben Alcázar