目次

1. Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay             Jonathan K. Fleming and Jonathan M. Wojciak   2. An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity             Melissa R. Pitman, Lorena T. Davies, and Stuart M. Pitson   3. Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells             Alena P. Chumanevich, Piper A. Wedman, and Carole A. Oskeritzian   4. Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry             Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, and Andrew J. Morris   5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines             Gary M. Reynolds, Barbara Visentin, and Roger Sabbadini   6. A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule             Jun-ichi Morishige, Ryouhei Yamashita, Tamotsu Tanaka, and Kiyoshi Satouchi   7. A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes             Naoki Kobayashi and Tsuyoshi Nishi   8. Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry             Jianhong Zhang, Annie Bang, and Stephen J. Lye   9. S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses             Camille Duran, Roland Kaunas, and Kayla Bayless   10. In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate             Melina G. Castro, Ludmila E. Campos, Yamila I. Rodriguez, and Sergio E. Alvarez   11. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor             Raymond C.B. Wong, Martin F. Pera, and Alice Pébay   12. Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors             Phillip Callihan, Mohammed Alqinyah, and Shelley B. Hooks   13. Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation             Guanghu Wang, Stefka D. Spassieva, and Erhard Bieberich   14. 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering             Shrestha Priyadarsini, Sarah E. Nicholas, and Dimitrios Karamichos