Erythropoiesis:Methods and Protocols (Methods in Molecular Biology, Vol. 1698) '17
目次
1. An Introduction to Erythropoiesis Approaches Joyce A. Lloyd 2. Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis Martin D. Kafina and Barry H. Paw 3. Mouse Models of Erythropoiesis and Associated Diseases Matthew P. Parker and Kenneth R. Peterson 4. Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells Skye C. McIver, Kyle J. Hewitt, Xin Gao, Charu Mehta, Jing Zhang, and Emery H. Bresnick 5. Stress Erythropoiesis Model Systems Laura F. Bennett, Chang Liao, and Robert F. Paulson 6. Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models Marie Trudel and Josepha-Clara Sedzro 7. Functional Analysis of Erythroid Progenitors by Colony-Forming Assays James Palis and Anne Koniski 8. Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands Jia Hao Yeo, Matthew P. Cosgriff, and Stuart T. Fraser 9. Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells Xiuli An and Lixiang Chen 10. Analysis of Erythropoiesis Using Imaging Flow Cytometry Theodosia Kalfa and Kathleen E. McGrath 11. Flow Cytometric Analysis of Erythroblast Enucleation Xiuli An and Lixiang Chen 12. High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure Alyson Smith, Roberta B. Nowak, and Velia M. Fowler 13. Chromatin Immunoprecipitation (ChIP) with Erythroid Samples Ivan Krivega and Ann Dean 14. Chromosome Conformation Capture (3C and Higher) with Erythroid Samples Ivan Krivega and Ann Dean 15. Genome Editing of Erythroid Cell Culture Model Systems Jinfen J. Yik, Merlin Crossley, and Kate G.R. Quinlan 16. In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood Anna Kovilakath, Safa Mohamad, Farrah Hermes, Shou Zhen Wang, Gordon D. Ginder, and Joyce A. Lloyd 17. Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines Divya S. Vinjamur and Daniel E. Bauer 18. Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products Neil W.A. McGowan, John D.M. Campbell, and Joanne C. Mountford
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