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Preface…Table of Contents…Contributing Authors…1. Diagnostic Imaging: UltrasoundStefan C. Kane, Su Lynn Khong, and Fabricio da Silva Costa2. Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the Cobas e 411 AnalyzerCarin Black and Fabricio da Silva Costa3. Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-EclampsiaKirsten Palmer4. Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-EclampsiaSebastian R. Hobson, Rebecca Lim, Joanne C. Mockler, Seshini Gurusinghe, and Euan M. Wallace5. Genetic Approaches in Pre-EclampsiaHannah E. J. Yong, Padma Murthi, Shaun P. Brennecke, and Eric K. Moses6. Epigenetics and Pre-Eclampsia: Programming of Future OutcomesAlberto Borges Peixoto, Liliam Cristine Rolo, Luciano Marcondes Machado Nardozza, and Edward Araujo Júnior7. Inflammatory and Immune System MarkersKelly J. McKelvey, Gaayathri Ariyakumar, and Sharon A. McCracken8. Methods to Enrich Exosomes from Conditioned Media and Biological FluidsShayna Sharma, Katherin Scholz-Romero, Gregory E. Rice, and Carlos Salomon9. Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental ExplantsMancy Tong and Larry W. Chamley10. Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal CirculationAndrew Lai, Omar Elfeky, Gregory E. Rice, and Carlos Salomon11. Proteomics Method to Identification of Protein Profiles in ExosomesVyjayanthi Kinhal, Andrew Lai, Zarin Nuzhat, Ramkumar Menon, Gregory E. Rice, and Carlos Salomon12. Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental ExplantsPriyadarshini Pantham and Larry W. Chamley13. Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion IndexSeverine A. Degrelle and Thierry Fournier14. Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target EffectsPaul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, and Padma Murthi15. Immunohistological TechniquesEvangelina Capobianco and Nora Martinez16. Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human OriginDominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice, and Carlos Salomon17. Isolation and Purification of Villous Cytotrophoblast Cells from Term Human PlacentaHélène Clabault, Laetitia Laurent, J. Thomas Sanderson, and Cathy Vaillancourt18. Analyzing Trophoblast Function Using Cell-Based AssaysKatie L. Powell and Anthony W. Ashton19. Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalisGina D. Kusuma, Mohamed H. Abumaree, Mark D. Pertile, and Bill Kalionis20. An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In VitroTejasvy Chollangi, Hélène Clabault, Andrée-Anne Hudon Thibeault, Hannah E. J. Yong, Shagun Narula, Ellen Menkhorst, J. Thomas Sanderson, Cathy Vaillancourt, and Padma Murthi21. In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental DiseasesLucas Sagrillo-Fagundes, Laetitia Laurent, and Cathy Vaillancourt22. Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse SystemDilys TH Leung and Simon Chu23. Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen BiosynthesisAndrée-Anne Hudon Thibeault, J. Thomas Sanderson, and Cathy Vaillancourt24. Placental Lipid TransportEvemie Dubé, Guillaume Desparois, and Julie Lafond25. EG-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal ModelDeborah Reynaud, Frédéric Sergent, Roland Abi Nahed, Sophie Brouillet, Mohamed Benharouga, and Nadia Alfaidy26. Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-EclampsiaBryan Leaw, Seshi Gurusinghe, Rebecca Lim, and Euan M. Wallace27. Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-EclampsiaSebastian R. Hobson, Rebecca Lim, and Euan M. Wallace28. A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP TrialSebastian R. Hobson, Euan M. Wallace, John C. Kingdom, and Ryan J. Hodges