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Preface…Table of Contents…Contributing Authors…1 Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris Raminta Venskutonytė, Karin Elbing, and Karin Lindkvist-Petersson2 Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3Dong Deng and Nieng Yan3 Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural StudiesGrégory Verdon, Hae Joo Kang, and David Drew4 GLUT Characterization using Frog Xenopus laevis OocytesWentong Long, Debbie O'Neill, and Chris I. Cheeseman5 Glucose Uptake in Heterologous Expression SystemsEunice E. Lee and Richard C. Wang6 Evaluating the Impact of GLUT Inhibition on Cellular Bioenergetics Using a Seahorse XF AnalyzerChangyong Wei, Monique Heitmeir, Paul W. Hruz, and Mala Shanmugam7 Glucose Transport Activity Measured in Giant VesiclesJesper Søndergaard Hansen and Karin Lindkvist-Petersson8 Design, Synthesis, and Evaluation of GLUT InhibitorsCarlotta Granchi, Tiziano Tuccinardi, and Filippo Minutolo9 Applying Microfluidic Systems to Study Effects of Glucose at Single Cell LevelNiek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, and Stefan Hohmann10 A Growth-Based Screening System for Hexose Transporters in YeastEckhard Boles and Mislav Oreb11 Identification of Insulin Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling TechniqueFrancoise Koumanov and Geoffrey D. Holman12 Total Internal Reflection Fluorescence Microscopy to Study GLUT4 TraffickingSebastian Wasserstrom, Björn Morén, and Karin G. Stenkula13 Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter AssayRobert M. Jackson and Ann Louise Olson14 GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by ImmunofluorescenceJavier R. Jaldin-Fincati, Philip J. Bilan, and Amira Klip15 Glucose Transport: Methods for Interrogating GLUT4 Trafficking in AdipocytesDougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, and James G. Burchfield16 Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking MachineryDimitrios Kioumourtzoglou, Gwyn W. Gould, and Nia J. Bryant17 Quantification of Cell Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeled AssayZahra Maria and Veronique A. Lacombe18 Tracking GLUT2 Translocation by Live Cell ImagingSabina Tsytkin-Kirschenzweig, Merav Cohen, and Yaakov Nahmias19 GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological AnalysisGwenaël Labouèbe, Bernard Thorens, and Christophe Lamy