目次

Part I: Experimental Model Systems 1. In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer Ka Yee Fung and Tracy Putoczki 2 Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer Sarah A. Best, Ariena Kersbergen, Marie-Liesse Asselin-Labat, and Kate D. Sutherland 3. Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer William Berry and Daniel Croagh 4. Modelling Intestinal Carcinogenesis Using In Vitro Organoid Cultures Thierry Jardé, Genevieve Kerr, Reyhan Akhtar, and Helen Abud 5. Protocols to Evaluate Cigarette Smoke-induced Lung Inflammation and Pathology in Mice Ross Vlahos and Steven Bozinovski 6. Tracking Competent Host Defence to Chronic Inflammation: An In vivo Model of Peritonitis Javier U. Fernandez, David Millrine, and Simon A. Jones 7. Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology Catherine L. Kennedy and Elizabeth L. Hartland 8. In Vivo Infection Model of Severe Influenza a Virus Ashley Mansell and Michelle D. Tate 9. In Vivo Models for Inflammatory Arthritis Gareth W. Jones, David G. Hill, Katie Sime, and Anwen S. Williams 10. Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease Le Son Tran and Richard L. Ferrero 11. Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer Christoph Garbers and Stefan Rose-John Part II:  Experimental Techniques 12. Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models Tae-Su Han and Masanobu Oshima 13. Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease Mohamed Saad and Saleela M. Ruwanpura 14. Quantifying Caspase-1 Activity in Murine Macrophages Dave Boucher, Amy Chan, Connie Ross, and Kate Schroder 15. Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, and Matthew P. McCormack 16. Analysis of DNA Methylation in Tissues Exposed to Inflammation Naoko Hattori and Toshikazu Ushijima 17. A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens Leonie A Cluse, Iva Nikolic, Deborah Knight, Piyush B Madhamshettiwar, Jennii Luu, Karla J Cowley, Timothy Semple, Gisela Mir Arnau, Jake Shortt, Ricky W Johnstone, and Kaylene J Simpson 18. Immuno-detection of Immature and Bioactive Forms of the Inflammatory Cytokine IL-18 Brendan J. Jenkins and Virginie Deswaerte 19. Optimisation Techniques for miRNA Expression in Low Frequency Immune Cell Populations Victoria G. Lyons, Natalie L. Payne, and Claire E. McCoy 20. Assessing the cGAS-cGAMP-STING Activity of Cancer Cells Genevieve Pepin and Michael Gantier 21. Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies Nicholas P.D. Liau and Jeffrey J. Babon 22. Production of Recombinant Killer Immunoglobulin-like Receptors for Crystallography and Luminex Based Assays Phillip Pymm and Julian P. Vivian 23. Isolation and Characterisation of Mouse Intrahepatic Lymphocytes by Flow Cytometry Florian Wiede and Tony Tiganis