Enzymes in Synthetic Biology (Methods in Enzymology, Vol. 608) '18
目次
Section I: Enzyme Discovery Enzyme Discovery: Enzyme Selection and Pathway Design P. Carbonell, Mathilde Koch, Thomas Duigou and J.L. Faulon Section II: Enzyme Engineering Tools and Platforms Cell-Free Synthetic Biology for Pathway Prototyping Ashty S. Karim and Michael C. Jewett Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution Joanna C. Sadler, Lucy Green, Neil Swainston, Douglas B. Kell and Andrew Currin Section III: Enzyme Families: Parts and Platforms for Chemical Diversity Sesquiterpene Synthase Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Non-natural Terpenoid Ether Florence Huynh David J. Miller and Rudolf K. Allemann In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity Guangkai Bian, Tian Ma and Tiangang Liu Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications S.C. Cosgrove, A. Brzezniak, S.P. France, J.I. Ramsden, J. Mangas-Sanchez, S.L. Montgomery, R.S. Heath and N.J. Turner Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins M.J.L.J. Fürst, C. Martin, N. Loncar and M.W. Fraaije Characterization of Cytochrome P450 Enzymes and their Applications in Synthetic Biology L. N. Jeffreys, H. M. Girvan, K. J. McLean and A. W. Munro Section IV: Genome Engineering for Enzyme Pathways Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion Carl Schultz, Jiazhang Lian and Huimin Zhao YeastFab: High Throughput Genetic Parts Construction, Measurement and Pathway Engineering in Yeast Eva Garcia-Ruiz, Jamie Auxillos, Tianyi Li, Junbiao Dai and Yizhi Cai Section V: Bioengineered Strains with Designed Enzyme Pathways Halomonas and Pathway Engineering for Bioplastics Production Jiang Xiao-Ran, Yin Jin, Chen Xiangbin and Chen Guo-Qiang An Engineering Approach for Rewiring Microbial Metabolism Sebastian Wenk, Oren Yishai, Steffen N. Lindner and Arren Bar-Even Multifragment DNA Assembly of Biochemical Pathways via Automated Ligase Cycling Reaction C.J. Robinson, M.S. Dunstan, N. Swainston, J. Titchmarsh, E. Takano, N.S. Scrutton and A.J. Jervis Commodity Chemicals From Engineered Multimodular Type I Polyketide Synthases Satoshi Yuzawa, Amin Zargar, Bo Pang, Leonard Katz and Jay D. Keasling Modular Pathway Rewiring of Yeast for Amino Acid Production Quanli Liu, Tao Yu, Kate Campbell, Jens Nielsen and Yun Chen
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