目次

Part I: Enzyme catalysis - a perspective.- Enzymes -Their place in Biology.- Enzymes - Historical aspects.- Exploiting enzymes – Technology.- On enzyme nomenclature and classification.- Hallmarks of an enzyme catalyst.- Origins of enzyme catalytic power.- Origins of enzyme catalytic power.- Which enzyme uses what trick? Some remarks.- Structure and catalysis: Conformational flexibility and protein motion.- Part II: Enzyme kinetic practice and measurements.- Chemical kinetics – Fundamentals.- Concepts of equilibrium and steady-state.- ES complex and pre-steady-state kinetics.- Principles of enzyme assays.- Good kinetic practices.- Quantification of catalysis and measures of enzyme purity.- Henri-Michaelis-Menten equation.- More complex rate expressions.- Enzyme kinetic data - Collection and analysis.- Part III: Elucidation of Kinetic mechanisms.- Approaches to kinetic mechanism – Overview.- Analysis of initial velocity patterns.- Enzyme inhibition analyses.- Irreversible inhibitions.- Reversible inhibitions.- Alternate substrate (product) interactions.- pH Studies with enzymes.- Isotopes in enzymology.- Isotope exchanges at equilibrium.- Isotope effects in enzymology.- From kinetic data to mechanism and back.- Part IV: Chemical mechanisms and catalysis.- Chemical reactivity and molecular interactions.- Acid-base chemistry and catalysis.- Nucleophilic catalysis and covalent reaction intermediates.- Phosphoryl group chemistry and importance of ATP.-  Enzymatic oxidation-reduction reactions.- Carboxylations and decarboxylations.- Electrophilic catalysis and amino acid transformations.- Integrating kinetic and chemical mechanisms – A synthesis.- Part V: Frontiers in enzymology.- Regulation of enzyme activity.- In vitro versus in vivo - Concept and consequences.- Future of enzymology – An appraisal.- Closure - Whither Enzymology.