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目次

Part 1 The cell: Plasma membrane - phospholipids, proteins, cholesterol: Prokaryotic outer barriers - cell wall (NAMs, NAGs, crosslinking); The cytoplasm - more proteins: enzymes (enzymes are catalysts, enzymes depend on proper protein folding, enzymes used in science, enzymes used in industry; The nucleus - organization of the genome (chromosomes, DNA fibers, nucleosomes, DNA strands, RNA); Prokaryotic genome organization - circular genome, attached to inner plasma membrane; The central dogma - transcription (mRNA); The life of a cell - the cell cycle (synthesis (S) phase, mitosis (M), gap phases, cell cycle control). Part 2 Biotechnology: Applications to and of the cell; Genetic engineering - genes (exons, introns, promoters, enhancers); Gene delivery models - viral (adenovirus, adeno-associated virus, herpesvirus, lentivirus/retrovirus); Gene delivery cargo - DNA, RNA, siRNA; Fluorescence - quantum yield, Stokes' shift, brightness; Recombinant Proteins; Biofuels - from cellulose degradation (biochemistry of cellulose); Stem cells - cells with potential (totipotent, pluripotent, multipotent); Transgenics; Cloning.