内容

目次

Ch 1. Genetics and Embryology of the Mouse: Past, Present, and Future Ch 2. Summary of Mouse Development Ch 3. A Mouse Colony for the Production of Transgenic and Chimeric Animals Ch 4. Recovery and In Vitro Culture of Preimplantation Embryos Ch 5. Isolation, Culture, and Manipulation of Postimplantation Embryos Ch 6. Surgical Procedures Ch 7. Production of Transgenic Mice by Pronuclear Microinjection Ch 8. Embryo-derived Stem Cell Lines Ch 9. Germ Line–Competent Stem Cells Derived from Adult Mice Ch 10. Vector Designs for Pluripotent Stem Cell-based Transgenesis and Genome Alterations Ch 11. Introduction of Foreign DNA into Embryonic Stem Cells Ch 12. Production of Chimeras Ch 13. Genotyping Ch 14. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning Ch 15. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection Ch 16. Cryopreservation, Rederivation, and Transport of Mouse Strains Ch 17. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems Ch 18. Setting Up a Micromanipulation Lab Appendices: Buffers & Solutions Web Resources Cautions