【ファ-ジディスプレイ】
Phage Display:A Practical Approach '04
内容
目次
1. Introduction to phage biology and phage display; 2. Constructingphage display libraries by oligonucleotide-directed mutagenesis; 3. In vitroDNA recombination; 4. Phage selection strategies for improved affinity andspecificity of proteins and peptides; 5. Rapid screening of phage displayedprotein binding affinities by phage ELISA; 6. Identification of novel ligandsfor receptors using recombinant peptide libraries; 7. Substrate phagedisplay; 8. Protease-based selection of stably folded proteins and proteindomains from phage display libraries; 9. Phage display of zinc fingers andother nucleic acid-binding motifs; 10. In vivo and ex vivo selections usingphage-displayed libraries; 11. Screening phage libraries with sera; 12.Interaction cloning using cDNA libraries displayed on phage; 13. Phageantibody libraries; 14. Affinity maturation of phage antibodies