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IntroductionThe immune response to infectious agentsGeneral MethodsPhenotyping and separation of leukocyte populations based on affinity labellingCytometric cytokine secretion assay: detection and isolation of cytokine secreting T cellsMeasurement of cellular proliferationUse of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine designThe use of tetramers in the quantitative analysis of T cell responsesUsing microarrays for studying the host transcriptional response to microbial infectionGenome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophagesMurine ModelsManagement of immuno-compromised and infected animalsIn Vitro AnalysisIsolation and preparation of lymphocytes from infected animals for in vitro analysisIsolation and development of murine T-lymphocyte hybridomas and clonal cell linesKiller cell assaysQuantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR MethodsIsolation of and measuring the function of professional phagocytes: murine macrophagesIn vivo AnalysisThe immune response in mice challenged with bacterial infectionsMeasuring immune responses in vivoSpecific ModelsMurine model of tuberculosisThe leishmaniasis modelAnimal models: murine cytomegalovirusDNA vaccines: fundamentals and practicePreparation and use of adjuvantsHuman SystemsIsolation and propagation of human dendritic cellsIsolation of T cells and establishment of T-cell lines and clonesGrowth-transformation of human T cellsGeneration and characterisation of human killer cellsMeasuring human cytokine responsesMeasuring immune responses in situ: immunofluorescent and immunoenzymatic techniquesIsolation, characterisation and cultivation ofhuman monocytes and macrophages