Groner, B 　著

内容

目次

PEPTIDES AS DRUGS: DISCOVERY AND DEVELOPMENT Discovery of New Potential Drug Targets and the Limitations of Druggability Protein Interaction Domains are at the Core of Signaling Pathways Peptides as Inhibitors of Protein Interactions MIMICS OF GROWTH FACTORS AND CYTOKINES Introduction The Cytokines Defining Receptor Recognition Sites in Cytokines Using Chimeric Proteins Receptor Recognition Sites are Organized as Exchangeable Modules The Concept of Fusing the Cytokine to the Soluble Receptor: Hyper–IL–6 Antagonists Specifically Inhibiting IL–6 Trans–Signaling In Vitro Evolution of Peptides and Proteins PEPTIDES DERIVED FROM EXON v& OF THE CD$$ EXTRACELLULAR DOMAIN PREVENT ACTIVATION OF RECEPTOR TYROSINE KINASE AND SUBSEQUENTLY ANGIOGENESIS AND METASTATIC SPREAD OF TUMOR CELLS Introduction CD44 Proteins and Their Involvement in RTK Activation CD44v6 Acts as a Coreceptor for c–Met and Ron Three Amino Acids in CD44 Exon v6 are Crucial for the CD44v6 Coreceptor Function, and Small Peptides Can Interfere with This Function The Ectodomain of CD44v6 Binds to HGF Peptides Corresponding to Exon v6 of CD44 Inhibit Metastatic Spread of Tumor Cells The Significance of the Collaboration Between CD44v6 and c–Met In Vivo The CD44v6 Peptides Interfere with Angiogenesis PEPTIDE APTAMERS TARGETING THE VIRAL E6 ONCOPROTEIN INDUCE APOPTOSIS IN HPV–POSITIVE CANCER CELLS Human Papillomaviruses and Oncogenesis Peptide Aptamers Targeting the HPV E6 Oncoprotein E6–Targeting Peptide Aptamers: Therapeutic Perspectives Perspectives THE PREVENTION OF HIV INFECTION WITH VIRAL ENTRY INHIBITORS Introduction: The Potential of Peptides as Drugs in the Treatment of HIV Infection The HIV Entry Process Peptides that Inhibit Receptor or Coreceptor Binding Inhibitors of the Viral and Cellular Membrane Fusion Process Entry Inhibitory Peptides Selected by the Phage Display Technology Limitations of Peptides it the Treatment of HIV Infection Strategies to Prolong the In Vivo Half–Life of Antiviral Peptides Antiviral Peptides in Gene Therapy of HIV Infection INTRACELLULAR EXPRESSION OF PEPTIDES Introduction Peptide Design and Expression Cassettes Stable Delivery and Expression of Peptides: Gamma–Retro– and Lentiviral Vectors Gamma–Retroviral Vectors Lentiviral Peptide Delivery Vectors for Transient Expression of Peptides: Adenoviruses and Adeno–Associated Viruses Perspective THE INTERNALIZATION MECHANISMS AND BIOACTIVITY OF THE CELL–PENETRATING PEPTIDES Introduction Discovery and Classification of CPPs Internalization Mechanisms of Cell–Penetrating Peptides Models of CPP Uptake The Current View of CPP Uptake CPPs as Cargo Delivery Vehicles Delivery of Proteins CPPs in Gene Delivery Delivery of Oligonucleotides Cytotoxicity of Cell–Penetrating Peptides In Vivo Drug Delivery with CPPs CPPs for Targeted Delivery PRODUCTION AND PURIFICATION OF MONOMERIC RECOMBINANT PEPTIDE APTAMERS: REQUIREMENTS FOR EFFICIENT INTRACELLULAR UPTAKE AND TARGET INHIBITION Introduction Protein Production Protein Purification Isolation of Monomeric, Natively Folded Proteins Increasing Peptide Production, Purification and Efficacy by Using Scaffolds The Use of Cell–Penetrating Peptides for Cellular Uptake of Purified Proteins Classification of Therapeutic Peptides Production and Administration of Therapeutic Peptides In Vivo PEPTIDE ARRAYS ON SOLID SUUPORTS: A TOOL FOR THE IDENTIFICATION OF PEPTIDE LIGANDS Introduction Synthesis of Peptide Arrays Applications of Peptide Arrays Challenges in High–Throughput Screening (HTS) Future Perspectives