Biomass, Part A: Cellulose and Hemicellulose.(Methods in Enzymology Vol. 160) cloth xxxv, 774 p.
目次
Cellulose. Preparation of Cellulosic Substrates: Increasing theAvailability of Cellulose in Biomass Materials. Cadoxen Solvolysis ofCellulose. Preparation of Crystalline, Amorphous, and Dyed CellulaseSubstrates. Preparation of Cellodextrins. Preparation of Cellodextrins:Another Perspective. Fluorogenic and Chromogenic Glycosides as Substrates andLigands of Carbohydrases. Use of Complex Formation between Congo Red andPolysaccharides in Detection and Assay of Polysaccharide Hydrolases.;Soluble, Dye-Labeled Polysaccharides for the Assay of Endohydrolases. Assaysfor Cellulolytic Enzymes: Methods for Measuring Cellulase Activities.Cellulase Assay Based on Cellobiose Dehydrogenase. Nephelometric andTurbidometric Assay for Cellulase. Selective Assay forExo-1,4-/-Glucosidases. Viscosimetric Determination of CarboxymethylcellulaseActivity. Staining Techniques for the Detection of the Individual Componentsof Cellulolytic Enzyme Systems. Chromatographic Methods for Carbohydrates:Liquid Chromatography of Carbohydrate Monomers and Oligomers. QuantitativeThin-Layer Chromatography of Sugars, Sugar Acids, and Polyalcohols.High-Performance Thin-Layer Chromatography of Starch, Cellulose, Xylan, andChitin Hydrolyzates. Miscellaneous Methods for Cellulolytic Enzymes:Screening of Prokaryotes for Cellulose- and Hemicellulose-Degrading Enzymes.Chromatographic Separation of Cellulolytic Enzymes. Morphological Aspects ofWood Degradation. Purification of Cellulose-Degrading Enzymes: Cellulases ofPseudomonas fluorescens var. cellulosa. Cellulases of Cellulomonas uda.Cellulase of Ruminococcus albus. Cellulase of Trichoderma koningii.Cellulases of Trichoderma reesei. Cellulases of a Mutant Strain ofTrichoderma viride QM 9414. Cellulases from Eupenicillium javanicum. GentaroOkada, Cellulase of Aspergillus niger. Cellulase and Hemicellulase fromAspergillus fumigatus Fresenius. Cellulases of Aspergillus aculeatus.Cellulases from Thermoascus aurantiacus. 1,4-/-*xD-Glucan Cellobiohydrolasefrom Sclerotium rolfsii. Cellulases of Thermomonospora fusca. Cellulases ofHumicola insolens and Humicola grisea. Cellulase-Hemicellulase Complex ofPhoma hibernica. Cellulases from Sporocytophaga myxococcoides. Cellulases inPhaseolus vulgaris. Endoglucanase from Clostridium thermocellum. CrystallineEndoglucanase D of Clostridium thermocellum Overproduced in Escherichia coli.Isolation of 1,4-/-*xD-Glucan 4-Glucanohydrolases of Talaromyces emersonii.Endo-1,4-/-Glucanases of Sporotrichum pulverulentum. Carboxymethylcellulasefrom Sclerotium rolfsii. /-Glucanases from Pisum sativum. Cellobiosidase fromRuminococcus albus. Cellobiohydrolases of Penicillium pinophilum.Exocellulase of Irpex lacteus (Polyporus tulupiferae). /-Glucosidase fromRuminococcus albus. 1,4-/-Glucosidases of Sporotrichum pulverulentum./-*xD-Glucosidases from Sclerotium rolfsii. Purification and Assay of/-Glucosidase from Schizophyllum commune. Purification of /-*xD-GlucosideGlucohydrolases of Talaromyces emersonii. Cellobiose Dehydrogenase fromSporotrichum thermophile. Cellobiose Dehydrogenase from Sclerotium rolfsii.Cellobiose Dehydrogenase Produced by Monilia. Cellobiose Dehydrogenase(Quinone). Cellobiose Phosphorylase from Cellvibrio gilvus. Cellulosomes fromClostridium thermocellum. Macrocellulase Complexes and Yellow AffinitySubstance from Clostridium thermocellum. Acid Proteases from Sporotrichumpulverulentum. Hemicellulose. Preparation of Substrates for Hemicellulases:Purification of (1-3),(1-4)-/-Glucan from Barley Flour. Synthesis of/-*xD-Mannopyranosides for the Assay of /-*xD-Mannosidase andExo-/-*xD-mannanase. Enzymatic Preparation of /-1,4-Mannooligosaccharides and/-1,4-Glucomannooligosaccharides. Carob and Guar Galactomannans. Xylobioseand Xylooligomers. Remazol Brilliant Blue-Xylan: A Soluble ChromogenicSubstrate for Xylanases. Preparation of *xL-Arabinan and 1,5-*xL-Arabinan.Analysis of /-Glucan and Enzyme Assays: Measurement of(1-3),(1-4)-/-*xD-Glucan. Measurement of Acetylxylan Esterase inStreptomyces. 4-O-Methyl-*ga-*xD-Glucuronidase Component of XylanolyticComplexes. Lichenase from Bacillus subtilis. Purification of/-*xD-Glucosidase from Aspergillus niger. Exo-1,4-/-Mannanase from Aeromonashydrophila.; Exo-/-*xD-mannanase from Cyamopsis tetragonolobus GuarSeed./-*xD-Mannanase. /-Mannanase of Streptomyces. /-*xD-Mannosidase from Helixpomatia. *ga-Mannanase from Rhodococcus erythropolis. *ga-*xD-Galactosidasefrom Lucerne and GuarSeed. Xylanase of Bacillus pumilus. Xylanase ofCryptococcus albidus. Xylanases of Streptomyces. Xylanases of AlkalophilicThermophilic Bacillus. Xylanase A of Schizophyllum commune. Xylanases and/-Xylosidase of Trichoderma lignorum. Xylanase of Malbranchea pulchella var.sulfurea. Xylanase of Talaromyces byssochlamydoides. 1,4-/-*xD-XylanXylohydrolase of Sclerotium rolfsii. /-Xylosidases of Several Fungi./-Xylosidase//-Glucosidase of Chaetomium trilaterale. Acetylxylan Esterase ofSchizophyllum commune. *ga-*xL-Arabinofuranosidase from Aspergillus niger.*ga-*xL-Arabinofuranosidase from Scopolia japonica. Arabinogalactanase ofBacillus subtilis var. amylosacchariticus. Author Index. Subject Index.