Quantitation of mRNA by Polymerase Chain Reaction 1995th ed.(Springer Lab Manuals) H XX, 165 S. 95
Köhler, Thomas,
Laßner, Dirk,
Rost, Anne-Katrin,
Thamm, Barbara,
Pustowoit, Barbara,
Remke, Harald
著
発行年月 |
1995年10月 |
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出版社/提供元 |
Springer-Verlag GmbH |
出版国 |
ドイツ |
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言語 |
英語 |
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媒体 |
冊子 |
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装丁 |
hardcover |
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ページ数/巻数 |
XX, 165 S. |
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ジャンル |
洋書/生命科学・医学/基礎医学/生化学 |
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ISBN |
9783540591924 |
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商品コード |
0209531409 |
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本の性格 |
学術書 |
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商品URL | https://kw.maruzen.co.jp/ims/itemDetail.html?itmCd=0209531409 |
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内容
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.