Harlow, E., Lane, D. 　著

内容

目次

Preface Dedication Chapter 1: Antibody Structure And FunctionChapter 2: Antibody-Antigen Interactions Chapter 3: Choosing AntibodiesChapter 4: Handling Antibodies Storing Antibodies PurifyingAntibodies Labeling Antibodies Chapter 5: Staining Cells MajorConstraints Choosing The Correct Antibody Protocols For ImmunostainingOf Tissue Culture Cells Variations On The Immunostaining ProcedureChapter 6: Staining Tissues Major Constraints Choosing the CorrectAntibody Immunostaining Tissues With Polyclonal Or Monoclonal AntibodiesProtocols For Staining Tissue Sections Variations For Staining YeastVariations For Staining Drosophila Protocols For Antigen RetrievalChapter 7: Immunoprecipitation Major Constraints Choosing The CorrectAntibody Immunoprecipitation Protocols Variations On TheImmunoprecipitation Procedure Chapter 8: Immunoblotting MajorConstraints Choosing The Correct Antibody Immunoblotting ProtocolsVariations In The Detection Methods Variations In The ImmunoblottingTechnique Chapter 9: Immunoaffinity Purification Major ConstraintsChoosing The Correct Antibody Immunoaffinity Purification With PolyclonalAnd Monoclonal Antibodies Protocols For Immunoaffinity PurificationVariations On Immunoaffinity Purification Chapter 10: Tagging ProteinsMajor Constraints Why Tag Your Protein Tags For Protein DetectionTags For Protein Purification Choosing The Right Tag Deciding Where ToPosition The Tag How To Tag Your Protein Chapter 11: Epitope MappingDetermining The Structural Requirements Of An Epitope Choosing AnEpitope-mapping Method Mapping By Competition Assay Mapping ByExpression Of Gene Fragments Mapping Using Synthetic Peptides SynthesisOr Purchase Of The Peptide Set Alternate Approaches To Epitope MappingAppendix I: Electrophoresis Appendix II: Protein Techniques AppendixIII: General Information Appendix IV: Cautions Appendix V: TrademarksAppendix VI: Suppliers