【モレキュラー・クローニング 第4版 全3巻】
Molecular Cloning:a Laboratory Manual, 4th ed. '12
Green, Michael R., Sambrook, Joseph 著
内容
目次
Contents: Volume 1 Part 1 Essentials DNA 1. Isolation and Quantification of DNA 2. Analysis of DNA CLONING 3. Cloning and Transformation with Plasmid Vectors 4. Gateway Recombinational Cloning John S. Reece-Hoyes and Albertha J.M. Walhout 5. Working with Bacterial Artificial Chromosomes and Other High-Capacity Vectors Nat Heintz and Shiaoching Gong 6. RNA: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells 7. PCR 8. Bioinformatics Jui-Hung Hung and Zhiping Weng Volume 2 Part 2 Analysis and Manipulations of DNA/RNA 9. Quantitation of DNA and RNA by Real-Time PCR 10. Nucleic Acid Platform Technologies Oliver Rando 11. DNA Sequencing Elaine Mardis and Richard McCombie 12. Analysis of DNA Methylation in Mammalian Cells Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee 13. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes 14. Methods for In Vitro Mutagenesis Matteo Forloni, Alex Liu, and Narendra Wajapeyee Part 3 INTRODUCING GENES INTO CELLS 15. Introducing Plasmid DNA into Cultured Mammalian Cells Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil 16. Viral Vectors for Introducing Genes into Mammalian Cells Guangping Gao and Miguel Sena-Esteves Volume 3 Part 4 Gene Expression 17. Analysis of Protein Expression in Cultured Mammalian Cells Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar 18. RNA Interference and Small RNA Analysis Chengjian Li and Phillip D. Zamore 19. Expressing Cloned Genes for Protein Production, Purification, and Analysis Clara L. Kielkopf, William Bauer, and Ina Urbatsch Part 5 Interaction Analysis 20. Cross-Linking Technologies for Analysis of Chromatin Structure and Function Tae Hoon Kim and Job Dekker 21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking Immunoprecipitation (CLIP) Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B. Darnell 22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays John S. Reece-Hoyes and Albertha J.M. Walhout Appendices 1. Reagents and Buffers Used in Molecular Cloning 2. Commonly Used Techniques 3. Detection Systems 4. General Safety and Hazardous Material Information Index
